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Indole-3-carbinol (I3C) and its metabolite DIM exert hormone-modulatory effects by shifting estrogen metabolism from the genotoxic 16α-hydroxyestrone pathway toward the less carcinogenic 2-hydroxyestrone pathway, enhancing CYP1A1/1A2 activity, and simultaneously downregulating ERα and upregulating ERβ expression, which together lead to suppression of estrogen-driven proliferation and sensitization of tumours to endocrine therapies (191)

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Its connection to gastric research helps explain why scientists later asked whether it might affect communication between the digestive system and the brain, often called the gut–brain axis