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Blots were washed three times with 1% (w/v) non-fat dry milk TTBS and incubated with the secondary antibody (rabbit for Id2 and mouse for α tubulin) diluted in 1% non-fat dry milk TTBS conjugated to horseradish peroxidase (Santa Cruz Biotechnology, CA, USA) for 60 min at room temperature
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Additionally, symptomatic blood flow recovery and reoxygenation can also cause damage, making the prevention of secondary cell deaths crucial (Eltzschig and Eckle, 2011)
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