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GHRH receptor agonism → adenylate cyclase activation Elevated cyclic AMP → growth hormone synthesis and release Position-2 substitution → resistance to dipeptidyl peptidase-4 cleavage Short half-life → pulsatile rather than sustained signaling Intact somatostatin feedback → preserved physiological regulation Core Areas of Scientific Investigation Pulsatile Release Physiology: Mod GRF 1-29 is used to generate discrete growth hormone pulses in study designs where pulse structure is the measured variable