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Western blot analyses using the iBLOT System (Invitrogen) were performed on nitrocellulose membranes after blocking in 1× Rotiblock (Carl Roth) in TBS-T using the fluorescence-labelled anti-BNIP3 antibody 1C8 (200 ng ml −1 ) and anti-BAX antibody (2D2, 1:1000, Cell Signaling Technologies) overnight
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