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Besides its apparent role in preserving mitochondrial function, a range of studies have also demonstrated the ability of ET to scavenge reactive oxygen and nitrogen species including hydroxyl radicals, singlet oxygen, and peroxynitrite (Halliwell and Cheah 2024

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As such, it is evident that betaine, like D-CYSee, is able to maintain redox homeostasis and protect mitochondria from opioid-induced toxicity via potential mechanisms that include the generation of methionine from homocysteine (Figure 3)