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Following purification, the proteins were buffer-exchanged into SEC buffer, and the volume was concentrated by ultrafiltration

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Otherwise, there is the milder increases of the pressure in the ligated superior sagittal sinus upon additional volume application in the right external jugular vein or in the inferior caval vein, where in the control rats, the portal/caval hypertension remained unchanged, but contributed to the further raise of the increased pressure in the superior sagittal sinus (Figure 5)