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As early as 3 weeks after AAV8-TBG- Cebpa dosing in HFCFD-fed C57BL/6N mice, hepatic Cebpa mRNA was increased, while hepatic Spp1 mRNA and serum OPN were decreased, in the absence of obvious phenotypic changes of biochemical endpoints and liver fibrosis (Supplemental Figure 10, I–P), supporting the view that CEBPA overexpression–mediated Spp1 downregulation could be an early causal factor for decreasing liver fibrosis

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This was demonstrated by the normalization of serum gonadotropins (LH, FHS) and testosterone, as well as the restoration of regular estrous cycles and enhanced ovarian morphology (Figure 2)