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Finally, to confirm the cell-type-specific dysregulation of these 34 overlapping genes, we compared their mRNA expression levels (RPKM) with those of their respective counterpart (iSPN − mut population) (Fig
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RNA Extraction and Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted from cellular and cerebral samples using TRIzol reagent (Invitrogen, Carlsbad, CA, United States)
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