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A checkerboard dose-response matrix of FSEN1 (0–15 μM) and RSL3 (0–15 μM) provided further evidence of synthetic lethality and indicated that the minimal doses that achieve maximal synergy for inducing ferroptosis in H460 C Cas9 cells are 0.55 μM FSEN1 together with 0.55 μM RSL3 (Figure 4B)