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To determine CI activity (NADH-dependent OCR), MAS plus alamethicin (2.5 μg ml −1 ) and cytochrome c (10 μg ml −1 ) was added onto the cells followed by 5 mM pyruvate + 5 mM malate + 1 mM NADH + 10 nM recombinant perfringolysin O at port A, 2 μM rotenone + 4 μM antimycin A at port B, 0.5 mM TMPD + 1 mM ascorbic acid at port C and 50 mM azide at port D
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