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a RNA-binding dCas, a trans-splicing donor construct comprising a guide portion, an intron, a splice acceptor, an exon of an endogenously expressed target pre-mRNA, a heterologous donor RNA, and a poly-A tail, wherein the guide portion is capable of forming a complex with the dCas and directing binding of the complex to an intron on a target pre-mRNA thereby facilitating splicing of the exon and the heterologous donor RNA into the target pre-mRNA to generate a modified mRNA comprising the heterologous sequences
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Normally, α-LA is found in small quantities in body tissues and is bound to an enzyme complex that limits its function as an antioxidant
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