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GSEA was performed on DS and euploid data combined from all ages and sexes, as neither age nor sex was previously observed to affect differential gene expression results

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While earlier studies suggested that nNOS is unlikely to be the mitochondrial NOS (mtNOS)46, subsequent studies suggest that mtNOS is nNOS or a spliced variant of it (likely nNOSα or nNOSμ), at least in the heart47