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Mass-by-difference is the weakest of these because the labeled mass includes counter-ion, residual water and residual salt, none of which is peptide, and the discrepancy is frequently in the tens of percent

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wherein: L 1a and L 1b are independently unsubstituted or substituted alkyl, L 2a and L 2b are independently absent, unsubstituted or substituted alkyl, unsubstituted or substituted aryl, or unsubstituted or substituted heteroaryl, L 3a and L 3b are independently absent or a linker moiety, m is an integer selected from the group consisting of 0-50, and P is an immunomodulating oligonucleotide