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In order to investigate whether elevated levels of reduced GSH were due to less redox active heme iron being liberated from impaired hemoglobin uptake and digestion in resistant parasites, 24 hpi trophozoite-stage parasites were treated for 3 h with 10 µM of the cysteine protease inhibitor, E64d, to block hemoglobin digestion and decrease free heme levels
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Along the same lines, interfering with sustained mutagenesis induced by the activation of the cytidine deaminase APOBEC3A in lung DTPs in response to targeted therapy might potentially delay the acquisition of drug resistance 189
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