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Using the genomic DNA, D1/D2 domain in the 28S rDNA was PCR-amplified for sequencing analysis using the specific primers NL-1 (5โ-GCATATCAATAAGCGGAGGAAAAG-3โฒ) and NL-4 (5โ-GGTCCGTGTTTCA AGACGG-3โฒ) (O'donnell, 1993)
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