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Cells were lysed with Triton-X-100 and lysed for 20 min on ice, then centrifuged at 1000× g for 10 min at 4 °C and supernatant was obtained
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Under normal aerobic conditions, most cells preferentially channel pyruvate into the mitochondrial tricarboxylic acid (TCA) cycle for complete oxidation and maximal ATP yield [21,22]
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