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ClinicalTrials.gov lists randomized, placebo-controlled trials of topical thymosin beta-4 in venous stasis ulcers (Phase 2, 72 participants, NCT00832091), of RGN-259 eye drops in dry eye (Phase 3, 700 participants, NCT03937882), and of intravenous recombinant thymosin beta-4 in healthy volunteers (Phase 1a, 54 participants, NCT04555824) and in heart attack (Phase 2, 90 participants, NCT05984134)

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Later, it was effectively used in ulcerative colitis trials (phase II) without adverse effects [63,64]