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Glutathione – The Super Antioxidant: Benefits and Role in Longevity
Under stress, GSH is oxidized to GSSG in the presence of glutathione peroxidase (GPX), while NADPH-dependent glutathione reductase (GR) is required for regeneration to GSH, but this enzyme also mediates the ascorbate–glutathione cycle
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Following the removal of the unbound antibody-enzyme reagent, 100 µL of streptoavidin–horseradish peroxidase (HRP) solution was added to the wells, which were then incubated for 20 min
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