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To normalise the levels of miRNA expression and other genes, U6 or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) RNA were utilised, and the relative gene expression levels were calculated using the 2−ΔΔCq method (38)
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1:100 in IF and 1:1000 in WB), mouse monoclonal ICP4 (Abcam
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