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Next day, the culture medium was removed and the cells were washed once with Krebs–Ringer phosphate (KRPG) buffer consisting of 145 mM NaCl, 5.7 mM sodium phosphate, 4.86 mM KCl, 0.54 mM CaCl 2 , 1.22 mM MgSO 4 , 5.5 mM glucose, pH 7.35)