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This improved base editing method enabled the successful execution of a small-scale loss-of-function screen, confirming the essential and/or growth-associated roles of several kinases (CK1.2, CRK2, CRK3, AUK1/AIRK, and TOR1), as well as proteins related to intraflagellar transport (IFT88, IFT139, and IFT140) and endocytosis (Rab5A)
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