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Barcode-based demultiplexing was performed using IDEMP software with default parameters ( Obtained reads were assembled, quality controlled, and clustered using Usearch8.1 software package ( Briefly, reads were merged using -fastq_mergepairs –with fastq_maxdiffs 30 and quality filtering was done with fastq_filter (-fastq_maxee 1), minimum read length 200 bp

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While this preservative enhances its utility for multi-dose applications, it also means it may not be suitable for certain patients, such as neonates or those with sensitivities to alcohol-based agents