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NHEK cells were maintained in Keratinocyte Basal Medium 2 (KBM2, PromoCell) supplemented with 0.4% bovine pituitary extract, 0.125 ng ml −1 rh EGF, 5 μg ml −1 rh insulin, 0.33 μg ml −1 hydrocortisone, 0.39 μg ml −1 epinephrine, 10 μg ml −1 transferrin and 0.06 mM CaCl 2

HPLC tested

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Azoles inhibit ergosterol biosynthesis and demonstrate excellent curative effects in mild-to-moderate mucosal infections (vulvovaginal, oral) with high oral bioavailability and good tolerance