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pH 7.5) containing protease-inhibitor mix HP (1:100 v/v) and benzonase (2 ΞΌL per 10 mL lysis buffer), and then incubated on ice for 30 min before sonication using an ultrasonic homogenizer (Sonoplus HD 2070, Bandelin, Berlin, Germany)
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Currently, the concentration of intracellular GSH is derived from either cell lysates or GSH- S -transferase (GST) dependent probes 7,8
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